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A Metabarcoding Protocol to Analyze Coastal Planktic Communities Collected by Desalination Plant Filters: From Sampling to Bioinformatic Exploratory Analyses

Chapter
Publication Date:
2022
abstract:
The temporal dynamics of coastal planktic communities can be disclosed through DNA metabarcoding on the filters of reverse-osmosis desalination plants. Here, we describe the steps that are necessary to process the filters in order to create the subsamples used for DNA extraction and the bioinformatic pipeline to perform the first exploratory analyses on this kind of dataset.
Iris type:
02.01 Contributo in volume (Capitolo o Saggio)
Keywords:
metabarcoding; monitoring
List of contributors:
Fontaneto, Diego; DI CESARE, Andrea; Eckert, ESTER MARIA
Authors of the University:
DI CESARE ANDREA
ECKERT ESTER MARIA
FONTANETO DIEGO
Handle:
https://iris.cnr.it/handle/20.500.14243/416884
Book title:
Marine genomics
Published in:
METHODS IN MOLECULAR BIOLOGY (CLIFTON N.J.)
Series
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URL

https://link.springer.com/protocol/10.1007/978-1-0716-2313-8_8#chapter-info
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