OMIP-079: Cell cycle of CD4⁺ and CD8⁺ naïve/memory T cell subsets, and of Treg cells from mouse spleen

A multicolor flow cytometry panel was designed and optimized to define the following nine mouse T cell subsets: Treg (CD3 CD4 CD8 FoxP3), CD4 T naïve (CD3 CD4 CD8FoxP3 CD44 CD62L), CD4 T central memory (CD3 CD4 CD8 FoxP3 CD44 CD62L), CD4 T effector memory (CD3 CD4 CD8 FoxP3 CD44 CD62L), CD4 T EMRA (CD3 CD4 CD8 FoxP3 CD44 CD62L), CD8 T naïve (CD3 CD8 CD4 CD44 CD62L), CD8 T central memory (CD3 CD8 CD4 CD44 CD62L), CD8 T effector memory (CD3 CD8 CD4 CD44 CD62L), and CD8 T EMRA (CD3 CD8 CD4 CD44 CD62L). In each T cell subset, a dual staining for Ki-67 expression and DNA content was employed to distinguish the following cell cycle phases: G (Ki67, with 2n DNA), G (Ki67, with 2n DNA), and S-G/M (Ki67, with 2n < DNA <= 4n). This panel was established for the analysis of mouse (C57BL/6J) spleen.