Effect of heat root stress and high salinity on glucosinolates metabolism in wild rocket

Wild rocket (Diplotaxis tenuifolia L.) is a leafy vegetable appreciated for its characteristic sensory properties which are mainly due to the presence of glucosinolates (GSLs). Short-term exposure to abiotic stresses can induce physiological responses and transcriptional changes which involve GSLs. For this reason, the aim of this work was to study the mechanisms of regulation of GSLs metabolism in rocket subjected to heat stress (40 °C) and high salinity (200 mM NaCl) imposed for up to 48 h. GSLs levels and the expression of methylthioalkylmalate synthase1 (DtMAM1), cytochromeP79F1 (DtCYP79F1), cytochromeP45083A1 (DtCYP83A1), cytosolic-sulfotransferase5b (DtST5b), cytosolic-sulfotransferase5c (DtST5c), flavinmono-oxygenase (DtFMO), myrosinase (DtMyro) and thio-methyl transferase (DtTMT) were analyzed under stress conditions. In addition, the effect on chlorophyll and glucose levels, as well as on chlorophyll a fluorescence were evaluated. Chlorophyll and chlorophyll fluorescence were not affected by the short-term application of stresses. Glucose levels in roots were doubled in response to high salinity, while, in the same organ, GSLs were three fold lower in response to both stresses. The relative content of several aliphatic GSLs was significantly reduced in leaves as a response to both stresses. A key role in GSLs metabolism and in the response to salinity is hypothesized for the gene DtTMT, as it showed an increment in transcripts accumulation (three-fold) consistent with the decrement in the GSLs levels found in salt-exposed leaves and roots. The results obtained in this study can be used in breeding programmes aiming to enhance rocket sensory quality and to improve the resistance to abiotic stresses.