D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor

D-Galactose/D-glucose-binding protein from E. coli (GGBP) is a monomer that binds glucose with high affinity. The protein structure of GGBP is organized in two principal domains linked by a hinge region that form the sugar-binding site. In this work we show that the mutant form of GGBP at the amino acid position 182 can be utilized as a probe for the development of a non-consuming analyte fluorescence biosensor to monitor the glucose level in diabetes health care.