Coltivazione di specie officinali: dalle colture in vitro convenzionali ai protocolli alternativi di propagazione in vitro per la realizzazione di colture

Here are reported the last advances in micropropagation protocols of MAPs. Lavandin (Lavandula officinalis L. X Lavandula latifolia L.) and mint (Mentha piperita L.) are characterized by a high incidence of shoot hyperidricity during in vitro culture. Trials has been done in order to study the influence of the physico-chemical components of the medium on the hyperidricity. A new protocol for the setting up of an axenic shoot cultures has been developed in Echinacea angustifolia L., since in this species it seems very difficult to reduce and to extinguish the contaminant level. With the attempt of shifting to photo-mixotrophic cultures, Passiflora incarnata L. in vitro cultures have been grown in a medium with reduced sucrose and mineral salts, measuring gas exchange (CO2 and ethylene) in different culture vessels. Since agriperlite has been used as artificial substrate, a new term "microponic" can be used.