Fabrication and Characterization of ALK1fc-Loaded Fluoro-Magnetic Nanoparticles for Inhibiting TGF ? in Hepatocellular Carcinoma (HCC)

Magnetic nanoparticles (MNPs) have shown imprortant properties to strength their use in medical applications for cancer therapy. They can be used as contrast agents in magnetic resonance imaging (MRI) (Terreno et al., 2010), vectors for drug delivery (Yoo et al., 2011) and mediators to convert electromagnetic energy to heat (Lee et al., 2011). The combination of magnetic and fluorescent properties in a single nano-system is important in medical bio-imaging as multi-functional contrast agents. Hence, attachment of fluorescence molecules such as fluorescence isothiocyanate (FITC) at the surface of iron oxide during fabrication is not produced yet. The purpose of this work is to produce Fluorescent Magnetic Nanoparticles (Fluoro-MNPs) coated by Layer by Layer (LbL) assembly to control anticancer drug delivery and to fabricate hydrogel cross-linked structure (PEG-CMC) onto fluoro-MNPs surface by using LbL assembled polymers for protein delivery .