Synthesis and biological evaluation of the disulfide form of the glutathione analogue g-(l-glutamyl)-l-cysteinyl-l-aspartyl-l-cysteine?

By using the chain to chain mode of cyclization the title glutathione analogue y-Glu-Cys-Asp-Cys (4), containing the 11-membered disulide ring –Cys-Asp-Cys- replacing the native -Cys-Gly fragment, has been synthesized and characterized together with its reduced dithiol form y-Glu-Cys-Asp-Cys (5). The activity of (4) with y-glutamyl-transferase and glutathione reductase has been evaluated and compared with those of the two conformationally restricted glutathione analogues (2) and (3) previously reported.