Fundamentals of enantioselective liquid chromatography

In this chapter, the basic developments and mechanisms of enantioseparations in high-performance liquid chromatography are summarized. In particular, the major type of chiral selectors and inert carriers used for the preparation of a chiral stationary phase are overviewed. The effects of mobile phases and mobile phase additives on a separation process are highlighted. Currently available tools for the thermodynamic treatment of the separation process, their advantages, and bottlenecks are discussed, and kinetic approaches are also briefly overviewed. The final part of the chapter deals with the experimental and theoretical tools currently in use for better understanding of chiral recognition mechanisms at the molecular level.