Probing the interaction interface of the GADD45 beta/MKK7 and MKK7/DTP3 complexes by chemical cross-linking mass spectrometry
Academic Article
Publication Date:
2018
abstract:
GADD45 beta is selectively and constitutively expressed in Multiple Myeloma cells, and this expression correlates with an unfavourable clinical outcome. GADD45 beta physically interacts with the JNK kinase, MKK7, inhibiting its activity to enable the survival of cancer cells. DTP3 is a small peptide inhibitor of the GADD45 beta/MKK7 complex and is able to restore MKK7/JNK activation, thereby promoting selective cell death of GADD45 beta-overexpressing cancer cells.
Iris type:
01.01 Articolo in rivista
Keywords:
GADD45 beta; MKK7; Protein-protein interaction; Chemical cross-linking; Mass spectrometry
List of contributors:
Ruvo, Menotti; Sandomenico, Annamaria
Published in: