Polymeric biocatalytic membranes with immobilized thermostable phosphotriesterase

In this work the catalytic performance of a mutant (named SsoPox W263F) of the thermophilic Phosphotriesterase-like lactonase (PLL) isolated from Sulfolobus solfataricus (SsoPox) was reported both on free and immobilized membrane systems using as substrate the pesticide paraoxon. The single mutant SsoPox W263F was immobilized on/into hydrophilic (Non standard grade Polyethersulphone, NGS-PES) and hydrophobic (Polyvinylidenefluoride, PVDF) membranes. Biocatalytic membrane systems able to work in liquid and vapour phase were developed aimed at decontamination of water and air, respectively. The work aimed at membranes characterization in terms of vapour and water permeability, free and immobilized enzyme catalytic and specific activity. Enzyme stability was also monitored for more than 5 months in order to evaluate the stability of developed systems.