LACTOSE DETERMINATION IN RAW MILK WITH A TWO-ENZYME BASED ELECTROCHEMICAL SENSOR

A hydrogen peroxide electrochemical sensor, coupled with two enzymes (.beta.-galactosidase and glucose oxidase) immobilized on the surface of the sensor covered with a cellulose acetate dialysis membrane (cut off 100), is proposed for the analysis of lactose in raw milk. The resulting hydrogen peroxide formation is measured with a platinum electrode polarized at +650 mV versus Ag/AgCl. The procedure developed is very simple and the short response time permits its use in assaying milk samples on dairy farms.