AFB1-AP conjugate for enzyme immunoassay of aflatoxin B1 in corn samples

This article describes the conjugation between aflatoxin B1 (AFB1), one of the major mycotoxins, and alkaline phosphatase (AP), one of the most used enzymes for immunoassays. In addition, an application of the ELISA method for aflatoxin B1 determination in corn is presented. Three AFB1-AP conjugates in different toxin-enzyme ratios were prepared and tested. The ELISA results, developed with the most effective conjugate obtained, showed a satisfactory working range between 2.4 and 4000 ng of toxin/g of corn. The detection limit was 2 ng/g in corn samples, and recoveries ranged from 105 to 120%