IMPROVEMENT OF HIGH FAT DIET-INDUCED HYPERLIPIDEMIA AND HYPERGLYCEMIA BY AN AQUEOUS EXTRACT OF LISOSAN® REDUCTION IN MICE

Lisosan® Reduction is a cereals and herbs mixture produced from powdered Triticum aestivum (Lisosan® G), Desmodium adscendens, Malus domestica, extract of rhizoma of Picrorhiza kurroa and seeds of Hordeum vulgare. The aim of this study was to evaluate the effects of aqueous extract of Lisosan® Reduction on mice fed a high fat diet. Thirty two C57BL/6J male mice were divided into 4 groups and fed with different diets for 11 weeks: control diet (CTR), control diet with 60 mg/kg b.w. of Lisosan® Reduction extract (CTR+Red), high fat diet (HFD) and high fat diet with 60 mg/kg b.w. of Lisosan® Reduction extract (HFD+Red). Serum AST, ALT, cholesterol, glucose and triglycerides content was measured with standard enzymatic techniques. Lipids were extracted from hepatic tissue adopting a gravimetric method while histological examination of liver section was performed by haematoxylin and eosin staining. The supplementation of aqueous Lisosan® Reduction extract in CTR+Red mice did not cause any alteration of serum AST, ALT, cholesterol, glucose and triglycerides level, hepatic lipids content and histological results, compared to CTR group. Otherwise, the supplementation with Lisosan Reduction in HFD+Red group showed a significant decrease of serum cholesterol (-27.6 %), glucose (-22.5 %) and triglycerides (-30.8 %) level, while no changes in serum AST and ALT concentration were detected, compared to HDF group. Moreover, HFD+Red mice did not display any improvement of hepatic lipid accumulation compared to HFD, confirmed by histological results. In conclusion, Lisosan® Reduction showed a hypocholesterolemic, hypoglycemic and hypotriglyceridemic effect diet but did not protect against steatosis in mice fed a high fat diet.