Peptide-based sensor and microfluidic platform for IgG antibody detection by differential impedance sensor

Serological assays enable infection screening as relatively easy-to-operate approaches compared with standard methods. In addition, to be relevant for early diagnosis, specific antibody detection is important for epidemiological surveillance and quantitative detection has potential significance for evaluating the severity and prognosis of different diseases. Here, we describe the detection process based on differential impedance sensing of IgG antibodies labeled with polystyrene nanoparticles. The electrode differential configuration, the amplification with nanoparticle functionalization, the electronic reading, and the microfluidic protocol allow to reach a limit of detection below 100 pg/mL for commercial IgG antibody spiked in buffer.