Effects of formalin preservation on eye lens of bogue, Boops boops (Linnaeus, 1758): Comparison of direct (post-fixation) vs ultrasound (true) measurements

The purpose of this study was to describe the normal ultrasonic appearance of eye lens samples from bogue cadavers by the mean of B-mode ultrasonography. In order to minimize decomposition processes the eye lenses were extracted one at a time from the fish eyes of 26 bogue (Boops boops) cadavers and immersed in 0.9% sodium chloride solution until scanning was performed. Both the eye lens were evaluated by B-mode ultrasonography immediately after collection. After examination the 52 eye lens were formal fixed air dried and equatorial eye lens diameter was measured to the nearest millimeter using a digital vernier caliper. High resolution ultrasound equipment produced high quality images and the difference between ultrasound and after fixation measurement resulted always highly significant.