Novel capacitance biosensor based on hafnium oxide for interleukin-10 protein detection

In this present study, we have analyzed one cytokine, interleukin-10 (IL-10), that plays a pivotal role in patients with chronic heart failure. For this purpose, a novel capacitance substrate, based on hafnium oxide (HfO2) grown by Atomic Layer Deposition on silicon, was applied to study the interaction between IL-10 with the corresponding antibody. HfO2 has been functionalized using an aldehyde monolayer, to directly immobilize the anti-human IL-10 monoclonal antibody. The interaction between antibody-antigen (Ab-An), was characterized by fluorescence patterning and electrochemical impedance spectroscopy (EIS). The preliminary results for fluorescence patterning, demonstrated bio-recognition of the recombinant protein, while Nyquist plots showed variation when we changed the concentration from 1-10 ng/mL. This demonstrated that the developed biosensor was sensitive to the detection of IL-10. (C) 2011 Published by Elsevier Ltd.