Characterization of plasma factors that alter the permeability to albumin withih isolated glomeruli

Focal segmental glomerulosclerosis (FSGS) is responsable for intractable proteinuria and has became the leading cause of renal insuffieciency in children. We fractionated serum from children with FSGS using two mixed chromatographic electrophoretic approaches and have purified ten proteins among several hundreds utilizing an isolated rat glomeruly assay. Six proteins were succesfully characterized by mass spectrometry.