Data di Pubblicazione:
2015
Abstract:
Genotyping wild and captive capuchins has become a priority and hair bulbs have high quality DNA. Here, we describe a method to non-invasively collect fresh-plucked strands of hair that exploits capuchins' manual dexterity and propensity to grasp and extract food. The apparatus consists of a transparent tube baited with food. Its extraction requires the monkey to place its forearm in contact with double-sided tape applied on the inner surface of the tube entrance. The "tube" method, successfully implemented with captive (N=23) and wild (N=21) capuchins, allowed us to obtain hair bulbs from most individuals and usable genomic DNA was extracted even from a single bulb.
Tipologia CRIS:
01.01 Articolo in rivista
Keywords:
hair collection; DNA; non-invasive method; Sapajus
Elenco autori:
Truppa, Valentina; Visalberghi, Elisabetta
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