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Ca2+ Imaging: Principles of Analysis and Enhancement

Chapter
Publication Date:
2010
abstract:
In this chapter, we review the theoretical and experimental foundations underling a quantitative approach to Ca2+ imaging, discuss equilibrium conditions and their violations and present a computational framework that can be used to estimate the spatial and temporal dynamics of Ca2+ signals based of fluorescence measurements with Ca2+ indicators.
Iris type:
02.01 Contributo in volume (Capitolo o Saggio)
Keywords:
Buffers; Law of mass action; Binding reactions; Fluorescent indicators; Optical measurement of Ca2+ concentration; Single wavelength and ratiometric imaging; Chemical equilibrium; Non-equilibrium conditions; Diffusion; Ion fluxes; Differential equations; Monte Carlo methods
List of contributors:
Bortolozzi, Mario; Mammano, Fabio
Handle:
https://iris.cnr.it/handle/20.500.14243/309255
Book title:
Calcium Measurement Methods, Neuromethods
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URL

http://www.amazon.co.uk/Calcium-Measurement-Methods-43-Neuromethods/dp/1607614758/ref=sr_1_2?ie=UTF8&s=books&qid=1257278556&sr=8-2
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