Morphological and molecular characterization of Longidorus danuvii sp. N. and L. silvae Roca, 1993 (Nematoda: Dorylaimida) from Serbia

Specimens of a Longidorus species, here described as L. danuvii sp. n., were recovered from the rhizosphere of Amorpha fruticosa growing in a floodplain forest on the bank of the river Danube at Novi Sad, Serbia; a second population was collected from Populus sp. also at Novi Sad. Longidorus danuvii sp. n. is characterised by medium sized body length (3.7-5.4 mm), slightly expanded, anteriorly flattened and laterally rounded lip region, set off by a depression from the rest of the body, distinctly bilobed amphidial pouches with lobes of about equal length, medium odontostyle length (86-101 µm) and elongate, conoid tail with more or less bent appearance. Four juvenile developmental stages were identified. A second Longidorus species, identified as L. silvae, was recovered from the rhizosphere of Quercus petraea at Rakovac, Fruška Gora mountain, Serbia. The discovery of two males is the second record for this species. PCR-RFLP analyses of the ITS containing regions and sequences of the D3 region of the 26S rDNA were done.