Electrochemically addressed biomolecules onto Au mu-array in a continuous flow mu-chamber

Preliminary results regarding electrochemical on-chip purification-deposition of proteins, for further multi-analyte biodetection based on multiplexed chronoamperometry, in a continuous flow mu-chamber are presented. The mu-chamber consists of a planar array of gold electrodes covered by a borofloat (R) glass reservoir provided with perpendicular plastic capillaries of 500 mu m internal diameter. The array of planar 7 x 7 working electrodes has been manufactured using optical lithography and chemical vapour deposition techniques. A his-tag alkaline phosphatase has been electrochemically addressed and its activity detected on a single electrode of the mu-array.