Effect of periodic feeding in sequencing batch reactor on substrate uptake and storage rates by a pure culture of Amaricoccus kaplicensis

A pure culture of Amaricoccus kaplicensis was aerobically cultured at a long culture residence time (Èc > 12 d), under periodic acetate feeding in a sequencing batch resctor (SBR). The cycle length and, correspondingly, the volumentric organic load rate (vOLR) were varied in the range 4-24 h and 0.76-0.12 g COD 1-1d-1, respectively. The transient response of the microorganism to the acetate spike was investigated throughout batch tests, as a function of SBR cycle length and vOLR. In all tested conditions, a rapid transient response was observed, mainly due to acetate storage in the form of polyhydroxybutyrate, since growth (production of active biomass) played a minor role. Apart from this general trend, the maximum rates under transient conditions increased as the cycle length increased from 4 to 24 h. In the SBR, the longest cycle also caused a decrease in floc size and settleability as well as an increase in the observed yield. The observed effect of SBR operating conditions on the physiological state of cells and their related transient response may have great significance on the performance of full scale activated sludge processes.A pure culture of Amaricoccus kaplicensis was aerobically cultured at a long culture residence time (Èc > 12 d), under periodic acetate feeding in a sequencing batch resctor (SBR). The cycle length and, correspondingly, the volumentric organic load rate (vOLR) were varied in the range 4-24 h and 0.76-0.12 g COD 1-1d-1, respectively. The transient response of the microorganism to the acetate spike was investigated throughout batch tests, as a function of SBR cycle length and vOLR. In all tested conditions, a rapid transient response was observed, mainly due to acetate storage in the form of polyhydroxybutyrate, since growth (production of active biomass) played a minor role. Apart from this general trend, the maximum rates under transient conditions increased as the cycle length increased from 4 to 24 h. In the SBR, the longest cycle also caused a decrease in floc size and settleability as well as an increase in the observed yield. The observed effect of SBR operating conditions on the physiological state of cells and their related transient response may have great significance on the performance of full scale activated sludge processes.