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The folding and aggregation properties of a single KH-domain protein: Ribosome binding factor A (RbfA) from Pseudomonas aeruginosa

Academic Article
Publication Date:
2021
abstract:
Methods: PaRbfA thermodynamic stability and folding mechanism: Far-UV CD and fluorescence spectroscopy, stopped-flow kinetics and chevron plot analysis, site-directed mutagenesis. Fibrils characterization: FT-IR spectroscopy, Thioflavin T fluorescence, Transmission Electron Microscopy (TEM) and X-ray fibrils diffraction.
Iris type:
01.01 Articolo in rivista
Keywords:
KH domains; Folding mechanism; Aggregation; Amyloid fibrils
List of contributors:
Ardit, Matteo; DI MATTEO, Adele
Authors of the University:
DI MATTEO ADELE
Handle:
https://iris.cnr.it/handle/20.500.14243/404932
Published in:
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
Journal
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