Human Neuronal SHSY5Y Cells on PVDF: PTrFE Copolymer Thin Films

Here, we investigate the in vitro biocompatibility of polyvinylidenefluoride (PVDF)-polytrifluoroethylene (PTrFE) copolymer thin films using neuronal cells. The aim is to obtain an optimal capping layer material for hybrid bio-organic electronic transducers based on organic electronic devices, which can be applied for bidirectional communication with the central nervous system. PVDF: PTrFE thin films were grown by spin coating and characterized by AFM. Human neuroblastoma SHSY5Y cells were used as neuronal model and their interaction with PVDF: PTrFE films was investigated in term of cell adhesion, proliferation, differentiation, and cytoskeleton organization. We show that PVDF: PTrFE thin films allowed standard SHSY5Y proliferation and neuronal differentiation, even if with reduced short-term cell adhesion and spreading.