Measurement of carbon dioxide hydration by carbonic anhydrase entrapped in submicrometer-sized nanoreactor.

This work describes a biosensor for direct measurements of carbon dioxide (CO2). The functionality of this device was tested by a QMB transducer coated by a liposome containing carbonic anhydrase (AC). During the CO2 adsorption process a change in the QMB frequency was observed. Another transducer was used in this context. By a high resolution temperature variation transducer, such as the LiTaO3. it has been possible to detect the heat generated during the reaction CO2 +H2O ->HCO3- + H+ developed inside the liposomes. In this work the response curves of both QMB and LiTaO3 are shown and commented.