Publication Date:
2001
abstract:
Eukaryal tRNA splicing endonucleases use the mature domains of pre-tRNAs as their primary recognition elements.
However, they can also cleave in a mode that is independent of the mature domain, when substrates are able to form the
bulge-helix-bulge structure (BHB), which is cleaved by archaeal tRNA endonucleases. We present evidence that the eukaryal
enzymes cleave their substrates after forming a structure that resembles the BHB. Consequently, these enzymes can cleave
substrates that lack the mature domain altogether. That raises the possibility that these enzymes could also cleave non-tRNA
substrates that already have a BHB. As predicted, they can do so, both in vitro and in vivo.
However, they can also cleave in a mode that is independent of the mature domain, when substrates are able to form the
bulge-helix-bulge structure (BHB), which is cleaved by archaeal tRNA endonucleases. We present evidence that the eukaryal
enzymes cleave their substrates after forming a structure that resembles the BHB. Consequently, these enzymes can cleave
substrates that lack the mature domain altogether. That raises the possibility that these enzymes could also cleave non-tRNA
substrates that already have a BHB. As predicted, they can do so, both in vitro and in vivo.
Iris type:
01.01 Articolo in rivista
Keywords:
system biology; biological networks; RNAomics and proteomics
List of contributors:
Baldi, MARIA IRENE; Fruscoloni, Paolo; TOCCHINI VALENTINI, GLAUCO PASQUALE
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