Terpene Profiles Composition and Micromorphological Analysis on Two Wild Populations of Helichrysum spp. from the Tuscan Archipelago (Central Italy)

Two wild populations of Helichrysum (Mill.) located at Elba Island (Tuscan Archipelago, Central Italy) were morphologically and phytochemically analyzed to taxonomically identify H. litoreum (population A) and H. italicum subsp. italicum (population B). Micromorphological and histochemical analyses were performed on the indumentum using Scanning Electron Microscope (SEM) and Light Microscope (LM). Morphometric analyses on vegetative and reproductive traits were also conducted. Finally, a chemotaxonomic analysis was carried out on the terpene profile of flowers, leaves and bark tissues using gas chromatography (GC-MS). Results suggested that morphological discriminant traits were mainly in leaves and cypselae glandular tissues. Phytochemical analysis indicated that a high relative content of ?-pinene and ?-caryophyllene were the main markers for population A, while a high relative content of neryl-acetate, ?-curcumene, isoitalicene and italicene, especially in the terpene profile of bark tissue, were the main compounds for discriminating population B. The analysis suggested that the wild population A could be mainly ascribed to H. litoreum, whilst population B is defined by H. italicum.