Characterisation of bacterial communities in indoor environment,

We investigated the presence of bacteria in a library environment and on stored books, performing morphological and molecular analyses. Isolated bacteria were characterised by PCR amplification of 16S rRNA locus and 16S-23S Intergenic Transcribed Spacer (ITS), followed by sequence determination and comparison. The results from SEM observation and molecular analysis showed the prevalence of bacteria belonging to the family Micrococcaceae, distinguished as Kocuria rosea, Arthrobacter nicotianae, Micrococcus luteus and Curtobacterium flaccumfaciens. Moreover, C. flaccumfaciens showed a high cellulolytic activity constituting a risk for paper artwork degradation.