Imaging and quantitative microscopy in turbid microfluidic channels by Digital Holography

Optical imaging of microscopic objects in transparent media is well assessed and deeply exploited [1]. However, if the object under analysis is dipped into a turbid medium, its imaging through conventional optics is hindered due to the strong scattering of the colloidal particles, thus severely degrading image quality. Even if initially the fluid is transparent, but becomes turbid by effect of a whatever chemical reaction or mixing, the processes cannot be observed. Here we show that Digital Holography is a reliable technique allowing a clear coherent imaging of the specimens through turbid fluids at Lab on a Chip scale, which is not achievable with any other imaging methods