An automatic real-time system for the determination of translational and rotational speeds of swimming micro-organisms

This paper describes a digital system designed for the automatic detection and measurement of the velocity of moving objects in images acquired by means of a common TV-camera mounted onto a microscope. The main real-time features are: . it can perform a real-time grey level difference between two successive frames in order to detect moving objects . it stores the images in the frame memory . it performs an automatic labelisation in order to recognise the moving micro-organisms . it calculates the area of the cells moving in the microscope field . it completes the analysis in few seconds.