Adjoint time domain method for fluorescent imaging in turbid media

Application of adjoint time domain methods to the inverse problem in 3D fluorescence imaging is a novel approach. We demonstrate the feasibility of this approach experimentally on the basis of a time gating technique completely in the time domain by using a small number of time windows. The evolution of the fluorescence energy density function inside a highly scattering cylinder was reconstructed together with optical paramaters. Reconstructed energy density was used in localizing two fluorescent tubes. Relatively accurate reconstruction demonstrates the effectiveness and the potential of the proposed technique. (C) 2008 Optical Society of America.