Increased expression of interleukin-32 in the inflamed ileum of ankylosing spondylitis patients

Methods. Quantitative gene expression analysis, by real-time PCR, of IL-32, IL-1 beta, IL-10, TNF-alpha and IFN-gamma was performed on ileal biopsies of 15 AS and 15 Crohn's disease (CD) patients and 10 healthy subjects (HSs). IL-32 tissue distribution was evaluated by immunohistochemistry. The effect of IL-32 on the production of IL-10 by intestinal epithelial cell lines was also evaluated.