Crystallization and preliminary X-ray diffraction studies of phospholipase D from Streptomyces sp.

rystals of purified phospholipase D (E.C. 3.1.4.4) from Streptomyces sp. strain PMF have been grown under two different crystallization conditions using vapour diffusion. Both conditions gave monoclinic crystals in space group P2(1). The unit-cell parameters were a = 57.28, b = 57.42, c = 68.70 Angstrom, beta = 93.17 degrees. The crystals diffract at 110 K to a resolution beyond 1.4 Angstrom using synchrotron radiation.