Publication Date:
2015
abstract:
In this paper we present an innovative, low cost and portable detection system for food quality control based on Thin Layer Chromatography method. The operating principle relies on the real-time monitoring of the chromatographic run through the measurement of the photocurrent induced in amorphous silicon (a-Si:H) photosensors by naturally fluorescent molecules present in the investigated sample. The integration in the system of the array of a-Si: H photosensor allows to achieve a sensitive and compact system without external optics for focusing the fluorescent light. The presented system was designed for detection and quantification of Ochratoxin A (OTA), a toxin due to food contamination by fungi. Tests for the detection of OTA were successfully performed achieving a minimum detectable quantity of 0.2 ng for both red wine extract and OTA standard solutions. Taking into account a 90 % of extraction efficiency and a volume of 2 mu l which can be spotted on the HPTLC plate, we infer that a volume of only 5.5 ml of red wine is necessary to compare OTA level with the law limit.
Iris type:
02.01 Contributo in volume (Capitolo o Saggio)
Keywords:
THIN-LAYER-CHROMATOGRAPHY; OCHRATOXIN; WINE
List of contributors:
Ricelli, Alessandra
Book title:
Amorphous Silicon Photosensors for Food Quality Control Applications