Apolipoprotein E and transferrin genotyping by ligation detection reaction and universal array

Here, we present the method that we have established for the simultaneous typing of the APOE and TF genes based on the ligation detection reaction (LDR)/universal array approach. The apolipoprotein E locus (APOE) is a known major susceptibility factor of AD disease. Among additional genetic loci associated with disease development, the transferrin gene (TF) has been suggested as a candidate locus for AD because it is the major transport protein for iron, which itself is an important factor in free-radical generation.