Publication Date:
2013
abstract:
The study of phytoplasma membrane protein interactions with host cell proteins is crucial to understand the life cycle of these unculturable microorganisms within their hosts. A step by step protocol for the heterologous expression of phytoplasma membrane proteins in Escherichia coli is described, together with a procedure to purify a suitable quantity of fusion antigen for successive application in the study of phytoplasma-host interactions.
Iris type:
02.01 Contributo in volume (Capitolo o Saggio)
Keywords:
Antigenic membrane protein; Escherichia coli; Histidine-tagged fusion protein; Immunodominant membrane protein; Prokaryotic expression vector
List of contributors:
Marzachi', Cristina; Galetto, Luciana
Book title:
Phytoplasma Methods and Protocols
Published in: