Chemical Reversibility and Stable Low-Potential NADH Detection with Nonconventional Conducting Polymer Nanotubule Modified Glassy Carbon Electrodes

Studies of the oxidation of â-nicotinamide adenine dinucleotide (NADH) at glassy carbon (GCEs) electrode surfaces, modified with nonconventional conducting polymer nanotubules, are reported. In contrast to the situation with conventional carbon electrodes, chemical reversibility of the NADH oxidation reaction was achieved by means of poly(1,2-diaminobenzene) conducting nanotubule coatings. A ¢Ep of 425 mV (vs Ag/AgCl; pH 7.0) was observed. The NADH amperometric response of the conducting nanotubule modified GCEs was shown to be extremely stable, with 98% of the initial response remaining after 48 h of stirring in the presence of 1 10-4 M NADH solutions (compared to 14% at the poly(1,2- diaminobenzene) modified GCEs). The nonconventional conducting polymer nanotubule-coated electrodes, when tested in amperometric mode for NADH electrochemical oxidation at an applied potential of 450 mV, showed a sensitivity of 99 nA/mM, an operational stability for 2 days, a storage stability of 2 weeks at 4 °C, a linearity from 5 10-5 to 1 10-3 M, and good NADH chemical reversibility, all of which make them useful tools for dehydrogenase enzyme probe assembly.