General experimental aspects for capillary electrophoretic analysis of gliadins in Triticum species.

Capillary electrophoresis (CE) of gliadins from Triticum species in acidic or isoelectric buffers is currently used. The performances of five buffers are compared here. The highest resolving power and the shortest time of analysis resulted associated to the isoelectric buffers. The slight different performance of the iminodiacetic acid (IDA) and aspartic acid (Asp) buffers systems should be taken into consideration before to start CE analyses. IDA buffer should be preffered when screening large germplasm collections or when a detailed characterisation of the omega-gliadins is required. Asp buffer proved to be the most satisfactory for cultivar discrimination or varietal identification.