A truncated driven Overhauser effect study of Adriamycin in water: Conformation of the glycosidic linkage

Truncateddriven nuclear Overhausereffect difference spectra have been used to ascertain the conformational characteristics of the glycosidiclinkage of Adriamycin in aqueous solution. The "two-spin approximation" has been employed to evaluate the cross-relaxation rates between nearby protons and to obtain the relative internuclear distances. The rotational angles ?1 = C(7)-O(7)-C(1?)-(H1?) and ?2 = H(7)-C(7)-O(7)-C(1?) have also been calculated.