NEW ANTIOXIDANT COMPOSITIONS OF 5-FLUOROURACYL AND ASCORBIC ACID IN PRESENCE OF HYDROXYCHAVICOL OR HYDROXYCHAVICOL DIMER

Antioxidant therapy is a modern method, effective in preventing the abnormal production of aggressive reactive oxygen species and limiting their harmful effects on the human body. In this regard, the treatment of a number of diseases is based on "traditional" drugs with targeted functionality and various antioxidants. Potential application of mixtures of phenolic antioxidants (bio-antioxidants) can be assessed in phytochemical therapies or in specific diets recommended for metabolic disorders. This study aims to create new effective bio-antioxidant compositions based on physiologically important compounds like ascorbic acid (AA) and natural or recently synthesized natural-like phenols as hydroxychavicol (HCh) and its dimer (DHCh) respectively, combined with 5-fluorouracyl (5-FU) - a common chemotherapy drug. Our previous investigations showed that 5-FU was not soluble in lipid substrate and the experiments of bulk lipid autoxidation in presence of 5-FU were made in presence of DMSO. Unfortunately, DMSO reduced the antioxidant potential of phenolic compounds. For that reason in this study we solved 5-FU in acetone solution of AA. Last was used as an amphiphilic compound because of its high solubility in water and in a lipid substrate at low concentrations (<0.3mM). The process was performed in a kinetic regime (in Petri dishes, with kinetically pure triacylglycerols of sunflower oil as lipid substrate, in the dark, at room temperature) in the presence of binary and/or ternary mixtures containing the substances mentioned above in different combinations. The presence of 5-FU did not affect the inhibition degree and the antioxidant reactivity of hydroxychavicol (HCh) and its dimer (DHCh) during the initial stage of the oxidation process.