Cigarette smoke increases TLR4 expression and modifies LPS mediated responses in airway epithelial cells

Contributo in Atti di convegno
Data di Pubblicazione:
2007
Abstract:
Airwayepitheliumisemergingasaregulatorofinnateimmuneresponsestoa
varietyofinsultsincludingcigarettesmoke.Themaingoalofthisstudywas
to exploretheeffectsofcigarettesmokeextracts(CSE)inthetolllikereceptor
(TLR) expressionandactivationinahumanbronchialepithelialcellline(16-
HBE). CSEincreasedtheexpressionofTLR4andtheLPSbinding,theNFkB
activation,thereleaseofIL-8andthechemotacticactivitytowardneutrophilsbut
did notinducetheTLR2expressionandtheERK1/2activation.LPSincreasedthe
expressionofTLR4andinducedbothNFkBandErk1/2activation.Thecombined
exposureof16-HBEtoCSEandLPS,furtherincreasedIL-8releaseandthe
chemotactic activitytowardneutrophils.Theseadditiveeffectswereassociated
to ERKactivationratherthantoNFkBactivation.Furthermore,CSEdecreased
the constitutiveIP-10releaseandcounteractedtheeffectofLPSininducing
IP-10 release.Inconclusion,cigarettesmokecontributestotheaccumulationof
neutrophils withintheairwaysofsmokersbyincreasingtheexpressionofTLR4
and bypreferentiallyorientatingTLR4activationtowardactivationofERKand
IL-8 production.
SupportedbyGlaxoSmithKline.
Tipologia CRIS:
04.01 Contributo in Atti di convegno
Elenco autori:
Autori di Ateneo:
Link alla scheda completa:
https://iris.cnr.it/handle/20.500.14243/2214
Pubblicato in:
EUROPEAN RESPIRATORY JOURNAL SUPPLEMENT
Journal