UVB-radiation-induced apoptosis in Jurkat cells: a coordinated fourier transform infrared spectroscopy -flow cytometry study

we studied the induction of apoptosis in Jurkat cells try
UVB radiation {wavelength 29s_!2t nm) at a dose of 310 mJi
cm?. We combined Fotrier transform infrared (FTIR) spectroscopy
with fow cytometry to determine whether the com'
binatisn of both techniques could provide new and imprnved
informaticn about celi modifications. To do this, n'e looked
for correspondences and correlations between spectroscopy
snd flory cytometry data and found three highly probable
spectrcscopic markers of apoptosis' The behavior of the wave
number strif't of troth the Amide I p-sheet componert and the
area of the 1083 cm I band reprcduced, with a high correlation,
the trehavinr of tke early apoptotic cell population,
while the trehavior of the Amide I area shorved a high correlatian
with the earty plus late apoptctic cell population'