Data di Pubblicazione:
2019
Abstract:
Abstract
Background: We previously examined expression of Long Interspersed Element-1 (LINE-1) in a variety of prostate
cancer cells including hormone-dependent LNCaP cells. These studies demonstrated expression and sub-cellular
localization of LINE-1 proteins, ORF1p, with antibody 4H1, and ORF2p, with antibody chA1-L1.
Results: Here we conduct immunoprecipitation/mass spectrometry analysis using chA1-L1 antibody against ORF2p
in LNCaP cells. Our results indicate that antigens detected by the antibody include the transcriptional regulator
BCLAF1. We show that chA1-L1 recognizes BCLAF1 using siRNA knockdown and overexpression of a tagged
BCLAF1. We also show that chA1-L1 antibody recognizes ORF2p in HEK293 cells overexpressing LINE-1. Further,
analysis of ORF2p (chA1-L1) and BCLAF1 foci using immunofluorescence in LNCaP cells showed significant
colocalization.
Background: We previously examined expression of Long Interspersed Element-1 (LINE-1) in a variety of prostate
cancer cells including hormone-dependent LNCaP cells. These studies demonstrated expression and sub-cellular
localization of LINE-1 proteins, ORF1p, with antibody 4H1, and ORF2p, with antibody chA1-L1.
Results: Here we conduct immunoprecipitation/mass spectrometry analysis using chA1-L1 antibody against ORF2p
in LNCaP cells. Our results indicate that antigens detected by the antibody include the transcriptional regulator
BCLAF1. We show that chA1-L1 recognizes BCLAF1 using siRNA knockdown and overexpression of a tagged
BCLAF1. We also show that chA1-L1 antibody recognizes ORF2p in HEK293 cells overexpressing LINE-1. Further,
analysis of ORF2p (chA1-L1) and BCLAF1 foci using immunofluorescence in LNCaP cells showed significant
colocalization.
Tipologia CRIS:
01.01 Articolo in rivista
Keywords:
BCLAF1; ORF2; ORF2 antibody; LINE-1; Prostate cancer
Elenco autori:
Spadafora, Corrado
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