Worldwide diffusion of Fig latent virus 1 in fig accessions and its detection by serological and molecular tools
Academic Article
Publication Date:
2010
abstract:
A virus with filamentous articles ca. 700 nm long, denoted Fig latent virus 1 (FLV-1) is widespread in Apulian
(southern Italy) fig orchards, in trees showing or not mosaic symptoms and in symptomless seedlings. The virus was
transmitted by sap inoculation to a very restricted range of herbaceous hosts without inducing apparent symptoms and
was transmitted through fig seeds to a very high percentage (80 to 100 %). It was successfully purified from root tissues of infected figs. A virus-specific antiserum raised in rabbits, proved useful for its detection in fig leaf dips by
immunosorbent electron microscopy (ISEM), Western Blot, dot immuno-binding (DIBA), ELISA.The viral genome
structure resembles that of members of the genus Trichovirus in the family Flexiviridae.
(southern Italy) fig orchards, in trees showing or not mosaic symptoms and in symptomless seedlings. The virus was
transmitted by sap inoculation to a very restricted range of herbaceous hosts without inducing apparent symptoms and
was transmitted through fig seeds to a very high percentage (80 to 100 %). It was successfully purified from root tissues of infected figs. A virus-specific antiserum raised in rabbits, proved useful for its detection in fig leaf dips by
immunosorbent electron microscopy (ISEM), Western Blot, dot immuno-binding (DIBA), ELISA.The viral genome
structure resembles that of members of the genus Trichovirus in the family Flexiviridae.
Iris type:
01.01 Articolo in rivista
Keywords:
fig latent virus; ELECTRON MICROSCOPY; Western blot; DIBA; ELISA.
List of contributors:
Castellano, Mariantonietta; Martelli, Giovanni; DE STRADIS, Angelo; Boscia, Donato; Minafra, Angelantonio
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