Morpho-molecular characterization of Ditylenchus gigas and D. oncogenus parasitizing broad bean, Vicia faba, in Algeria

A nematode survey conducted in 2013 in
Algeria, revealed the presence of a normal and giant races
of D. dipsaci species complex on broad bean, Vicia faba L.
In Algeria, the stem and bulb nematode causes severe
economic damage; however, no studies have been conducted
for accurate identification of the species and races
of nematode infecting faba bean fields, which make management
strategies, quarantine measures and phytosanitary
actions less efficient. The present study describes the morphological
and molecular analyses of two stem nematode
populations from Biskra and Guelma regions in Algeria.
The results revealed the presence of D. gigas and
D. oncogenus in Algeria. The latest one is identified for
the first time on Vicia faba as new host plant. The morphology
of both populations is similar to other D. gigas
and D. oncogenus populations from southern Italy.
Molecular phylogeny by using the ITS-5.8S-ITS2 region
clearly supported the presence of D. gigas and
D. oncogenus in Algeria.