Myotonic dystrophy: antisense oligonucleotides inhibition of DMPK gene expression in vitro
Academic Article
Publication Date:
1996
abstract:
Antisense phosphorothioate oligonucleotides, targeted against the first codon starting region of DMPK
mRNA, were successfully used in K562 and HepG2 cells to decrease DMPK expression. The most effective
antisense oligo, MIO1, when added to K562 cells, shows a 75% reduction of the DMPK gene expression 6 hours
after addition. The same molecule, when encapsulated in liposomes, delays myotonin mRNA decrease at 24
hours after cell treatment. This considerable success with such inhibition in vitro could be utilised to generate
a cell model to study myotonic dystrophy (DM) chemio-physiological alterations.
mRNA, were successfully used in K562 and HepG2 cells to decrease DMPK expression. The most effective
antisense oligo, MIO1, when added to K562 cells, shows a 75% reduction of the DMPK gene expression 6 hours
after addition. The same molecule, when encapsulated in liposomes, delays myotonin mRNA decrease at 24
hours after cell treatment. This considerable success with such inhibition in vitro could be utilised to generate
a cell model to study myotonic dystrophy (DM) chemio-physiological alterations.
Iris type:
01.01 Articolo in rivista
List of contributors:
Iacomino, Giuseppe
Published in: