Publication Date:
2017
abstract:
The pathway of ochratoxin A (OTA) biosynthesis has not yet been completely elucidated. Essentially, two kind of genes have been demonstrated to be involved in the biosynthesis of OTA. One of them is the nrps
gene encoding a non-ribosomal peptide synthetase (NRPS) which catalyzes the ligation between the isocoumarin group, constituting the polyketide group of OTA molecule, and the amino acid phenylalanine. Here we describe a conventional PCR method developed for the detection of OTA-producing molds belonging to Penicillium and Aspergillus genera by Luque et al. (Food Control 29:270-278, 2013). This method is based on the OTA nrps gene of Penicillium nordicum . It produces a specifi c amplicon of 459 bp and its functionality in naturally infected samples was also demonstrated.
gene encoding a non-ribosomal peptide synthetase (NRPS) which catalyzes the ligation between the isocoumarin group, constituting the polyketide group of OTA molecule, and the amino acid phenylalanine. Here we describe a conventional PCR method developed for the detection of OTA-producing molds belonging to Penicillium and Aspergillus genera by Luque et al. (Food Control 29:270-278, 2013). This method is based on the OTA nrps gene of Penicillium nordicum . It produces a specifi c amplicon of 459 bp and its functionality in naturally infected samples was also demonstrated.
Iris type:
02.01 Contributo in volume (Capitolo o Saggio)
Keywords:
Ochratoxin A; PCR detection; Biosynthetic gene; nrps; Food commodities; OTAproducing molds; Aspergillus and Penicillium genera
List of contributors:
Perrone, Giancarlo; Gallo, Antonia
Book title:
Mycotoxigenic Fungi: Methods and Protocols
Published in: