Data di Pubblicazione:
2018
Abstract:
Two main precautions must be taken into account to obtain high-resolution morphological and nanomechanical
characterization of biological specimens with an atomic force microscope: the tip-sample interaction
and the sample-substrate adhesion. In this chapter we discuss the necessary steps for a correct
preparation of three types of biological samples: erythrocytes, bacteria, and osteoblasts. The main goal is
to deliver reproducible protocols to produce good cellular adhesion and minimizing the morphological
alterations of the specimens.
characterization of biological specimens with an atomic force microscope: the tip-sample interaction
and the sample-substrate adhesion. In this chapter we discuss the necessary steps for a correct
preparation of three types of biological samples: erythrocytes, bacteria, and osteoblasts. The main goal is
to deliver reproducible protocols to produce good cellular adhesion and minimizing the morphological
alterations of the specimens.
Tipologia CRIS:
02.01 Contributo in volume (Capitolo o Saggio)
Keywords:
Atomic force microscopy; Bacteria; Living cells; Nanomechanical information; Topography; Young's modulus
Elenco autori:
Dinarelli, Simone; Girasole, Marco; Longo, Giovanni
Link alla scheda completa:
Titolo del libro:
Nanoscale Imaging: Methods and Protocols
Pubblicato in: