High-yielding enzymatic alpha-glucosylation of pyridoxine by marine alpha-glucosidase from Aplysia fasciata

We recently succeeded in the identification and
purification of an interesting marine exo-a-glucosidase
(EC 3.2.1.20) from the anaspidean mollusc
Aplysia fasciata. The enzyme was characterized by
good transglycosylation activity toward different
acceptors using maltose as donor. High-yielding
enzymatic a-glycosylation of pyridoxine using this
marine enzyme is reported here; the reaction has
been optimized, reaching 80% molar yield of products
(pyridoxine monoglucosides 24 g/l; pyridoxine
isomaltoside 35 g/l). High selectivity toward the 50
position is observed for both monoglucoside and
disaccharide formation. This is the first report
describing the enzymatic production of pyridoxine
isomaltoside.