Data di Pubblicazione:
2009
Abstract:
The interaction of the surface plasmons of gold nanoparticles (NP) a few nanometers in size with fluorophores
can be used to engineer their fluorescence properties. This possibility can be exploited in principle to obtain
nanodevices for protein-protein recognition. We studied different types of constructs based on gold NPs on
which derivatives of fluorescein were bound. The interaction of this fluorophore with the gold surface plasmon
resonances, mainly occurring through quenching, affects its excited-state lifetime that is measured by
fluorescence burst analysis in standard solutions. The binding of proteins to the gold NPs through
antigen-antibody recognition further modifies the dye excited-state lifetime. This change can therefore be
used to measure the protein concentration. The data reported here indicate that one can measure the
concentration of bovine serum albumine in solution with an apparent limit of detection of 5 +/- 2 pM.
can be used to engineer their fluorescence properties. This possibility can be exploited in principle to obtain
nanodevices for protein-protein recognition. We studied different types of constructs based on gold NPs on
which derivatives of fluorescein were bound. The interaction of this fluorophore with the gold surface plasmon
resonances, mainly occurring through quenching, affects its excited-state lifetime that is measured by
fluorescence burst analysis in standard solutions. The binding of proteins to the gold NPs through
antigen-antibody recognition further modifies the dye excited-state lifetime. This change can therefore be
used to measure the protein concentration. The data reported here indicate that one can measure the
concentration of bovine serum albumine in solution with an apparent limit of detection of 5 +/- 2 pM.
Tipologia CRIS:
01.01 Articolo in rivista
Elenco autori:
Tallarida, Graziella
Link alla scheda completa:
Pubblicato in: